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KMID : 0380219930260050426
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 5 p.426 ~ p.432
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Effect of Ethanol on the Fluidity of Plasma Membrane Vesicles Isolated from Cultured Mar 18.5 Hybridoma Cells
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Il Yun
In-Kyo Chung and Jung-Sook Kang
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Abstract
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Intramolecular excimer formation of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effects of ethanol on the rate and the range of lateral mobility and the range of rotational mobility of bulk bilayer structures of plasma membrane vesicles (ATCC-PMV) isolated from cultured hybridoma cells (ATCC TIB 216). In a concentration-dependent manner, ethanol increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the ATCC-PMV and decreased the anisotropy (r), the limiting anisotropy (r∞), and the order parameter (S) of DPH in the ATCC-PMV. This indicates that ethanol increased both the lateral and the rotational diffusion of the probes in the ATCC-PMV. Selective quenching of DPH by trinitro-phenyl groups was used to examine the range of the transbilayer asymmetric rotational diffusion of the ATCC-PMV. The anisotropy (r), the limiting anisotropy (r∞), and the order parameter (S) of DPH in the inner monolayer were 0.024, 0.032, and 0.069, respectively, greater than calculated for the outer monolayer of the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was also used to examine the transbilayer asymmetric effects of ethanol on the range of the rotational diffusion of the ATCC-PMV. Ethanol had a greater fluidizing effect on the outer monolayer as compared to the inner monolayer of the ATCC-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the ATCC-PMV.
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